Office Hours

Why does hybrid female in c not have any CD4 T cells?

• Is the MHC I overall structure the same, but the amino acids differ? what about the MHC II?

• explain the role of leader sequences, do they get spliced out? for example we have V4D1J3, does V1-3 get spliced out or are they apart of leader sequence so they do not? I am just confused about what segments get cut out of mRNA as introns.

• if you have a secreted IgM molecule that class switches to IgE that is a membrane bound antibody, does it go "back" to become membrane bound?

• help me better understand restriction enzymes. I get that they cut (and technically a fragment can only become smaller not longer when a RE comes in and snips snips). but whats the point? to amplify a smaller region of DNA?

• slide 7 shows gel electrophoresis but it shows that both no recombination vs recombination genes have the same size? but only when you do southern blot do we see a different pattern btw the two groups

• explain to me slide 10

• what does the professor mean by we will only see activating signals when we deal with healthy cells (but still in lower concentrations?)

• what do we need to know for cytokine receptor structure? he mentioned that if we knock out something in the receptor, what would happen overall?