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if you have a secreted IgM molecule that class switches to IgE that is a membrane bound antibody, does it go "back" to become membrane bound?

help me better understand restriction enzymes. I get that they cut (and technically a fragment can only become smaller not longer when a RE comes in and snips snips). but whats the point? to amplify a smaller region of DNA?

slide 7 shows gel electrophoresis but it shows that both no recombination vs recombination genes have the same size? but only when you do southern blot do we see a different pattern btw the two groups

explain to me slide 10